目的 建立12批火把花根片的超高效液相色谱(UPLC)指纹图谱,评价其批间质量一致性,并对主要化学成分进行鉴定,以明确其物质基础。方法 采用Waters CORTECS T3色谱柱(2.1 mm×100 mm,1.6 μm),乙腈-0.1%甲酸水溶液为流动相,梯度洗脱,流速为0.4 mL·min-1,检测波长为260 nm,柱温为35 ℃。利用“中药色谱指纹图谱相似度评价系统(2012版)”进行相似度分析。采用超高效液相色谱四极杆串联飞行时间质谱(UPLC-Q-TOF-MS)技术对火把花根片的主要成分进行鉴定。结果 建立的火把花根片UPLC指纹图谱共标定了27个共有峰,12批样品的相似度为0.992~0.999,鉴定了18个主要成分,多数为黄酮和酚苷类成分。结论 火把花根片批间质量一致性较好,所含成分的极性相对较大,与该制剂水提醇沉的生产工艺相关。本研究中建立的指纹图谱和主要成分的结构鉴定可为后期该品种的质量控制研究提供依据。
Abstract
OBJECTIVE To evaluate the inter-batch quality consistency of 12 batches of Huobahuagen tablets using UPLC fingerprints, and to identify the main chemical constituents for clarifying their substance bases. METHODS Chromatographic separation was performed on a Waters CORTECS T3 column (2.1 mm×100 mm, 1.6 μm). Acetonitrile-0.1% formic acid aqueous solution was adopted as mobile phase for gradient elution. The flow rate was set at 0.4 mL·min-1. The detection wavelength was set at 260 nm. The column temperature was maintained at 35 ℃. The similarity of fingerprint was evaluated by the “Similarity Evaluatio System of Chromatographic Fingerprint of Traditional Chinese Medicine” (2012 edition). At the same time, the main chemical constituents of Huobahuagen tablets were identified by UPLC-Q-TOF-MS method. RESULTS In the established fingerprint, a total of 27 common peaks were calibrated. The similarity of the tested samples was 0.992-0.999. Eighteen main constituents were identified, most of which were phenolic glycosides and flavonoids. CONCLUSION Huobahuagen tablets have good inter-batch quality consistency. The main components contained in this preparation have relatively high polarity, which is due to the production process of water extraction and alcohol precipitation. The results presented above can serve as a foundation for a later quality control study of this preparation.
关键词
火把花根片 /
超高效液相色谱指纹图谱 /
相似度分析 /
质量一致性 /
成分鉴定 /
物质基础
{{custom_keyword}} /
Key words
Huobahuagen tablet /
UPLC fingerprint /
similarity /
inter-batch consistency /
component identification /
substance bases
{{custom_keyword}} /
中图分类号：
R282
{{custom_clc.code}}
({{custom_clc.text}})
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] LI S Y, ZHANG C, HE L, et al. Clinical application of Tripterygium Hypoglaucum[J]. Chin J Ethnomed Ethnopharm (中国民族民间医药杂志), 2006,(83):325-330.
[2] WAN P, WANG H B, WANG H Y, et al. The chemical combosition, pharmacologic action and clinical application of Tripterygium hypoglaucum Huteh (TH)[J]. J Dermatol Venereol (皮肤病与性病), 2000, 22(4):20-21.
[3] WANG Y, LONG C F. The Advances in Chinese Medical Herbs (Triptergium hypoglaucum hutch)[J]. Med Recapit (医学综述), 2006, 12(11):691-692.
[4] YANG X H, ZHANG W C, CHEN X. Research overview of Colguhoumia root[J]. Clin J Tradit Chin Med (中医药临床杂志), 2007, 19(3):315-317.
[5] WANG L, XIANG F. Clinical application of Huobahuagen tablets[J]. Lishizhen Med Mater Med Res (时珍国医国药), 2002, 13(9):562-563.
[6] YIN C H, WU K L. Clinical application of Huobahuagen tablets[J]. J Chengde Med Coll (承德医学院学报), 2004, 21(1):42-44.
[7] ZHANG H, YANG J, GAO R X, et al. The therapeutic effect of Huo Ba Hua Gen tablets on 40 cases of rheumatoid arthritis is related to the inhibition of inflammatory reaction[J]. Prog Anat Sci (解剖科学进展), 2020, 26(3):251-254.
[8] GE J D, HUANG N N, LI X J Y, et al. Study on efficacy network and mechanism of Huobahuagen Tablets “treating different diseases with same method” for lupus erythematosus and rheumatoid arthritis[J]. Chin Tradit Herb Drugs (中草药), 2020, 51(16):4223-4235.
[9] ZHONG Y, ZHAO X, ZHAO J Q, et al. Research progress of pharmacological effects and clinical applications of Huobahuagen Tablets[J]. Drug Eval Res (药物评价研究), 2021, 44(9):2020-2027.
[10] LI X Y, FANG W J. Clinical observation on 60 cases of IgA nephropathy treated with Huobahuagen Tablets[J]. Chin Tradit Herb Drugs (中草药), 2021, 32(9):825-826.
[11] LI M Y. Analysis of 70 clinical cases of massive proteinuria of diabetic nephropathy under the treatment of Huoba Huagen tablet[J]. Chin J Hosp Pharm (中国医院药学杂志), 2014, 34(20):1762-1764.
[12] ZHOU J B, WANG L J, SUN X Y, et al. Comparison of efficacy of colquhounia root tablets and irbesartan tablets in treatment of DN[J]. Med J Nat Def Forces Southwest China (西南国防医药), 2016, 26(9):961-963.
[13] LI H B, WU S, CHEN L, et al. Short-term clinical observation of huobahuagen in the treatment of 50 cases of psoriasis[J]. J Clin Dermatol (临床皮肤科杂志), 2002, 31(12):773-774.
[14] YAN H B, CHEN Y, CHEN Q H. Analysis of curative effect of Huobahuagen tablets and Hengsai in treating chronic urticaria[J]. China J Lepr Skin Dis (中国麻风皮肤病杂志), 2007, 23(3):256-257.
[15] ZHANG X. Reaserch advance in toxicity of tripterygium preparatum and the attenuation method[J]. Chin Pharm J (中国药学杂志), 2013, 48(22):1897-1901.
[16] ZOU C C, YAN H Y. Research progress on chromatographic fingerprint similarity evaluation method for traditional Chinese medicine in the past 30 years( 1988-2017) and its prospect[J]. China J Chin Mater Med (中国中药杂志), 2018, 43(10):1969-1977.
[17] YAN H Y, ZOU C C. Progress and prospect of application of traditional Chinese medicine fingerprint (specific chromatogram) in Chinese Pharmacopoeia (2010-2020)[J]. J South Med Univ (南方医科大学学报), 2022, 42(1):150-155.
[18] ZHENG D D, ZHANG X M, YANG Y, et al. Study on HPLC fingerprints of Huobahuagen Tablets intermediate[J]. J China Pharm (中国药房), 2017, 28(33):4714-4717.
[19] LI C J, ZHOU X, ZHANG X M, et al. Studies on chemical constituents of Colquhoumia root aqueous extract, The 10th National Natural organic Chemistry Conference of Chinese Chemical Society[C]. Guangzhou: Chinese Chemical Society, 2014:96.
[20] LIU Y, WANG Y X. Analysis of Lu Mountain Clouds-Mist Tea′s composition and authenticity discrimination based on UPLC-QTOF-MS[J]. J Chin Inst Food Sci Technol (中国食品学报), 2020, 20(7):269-277.
[21] FU S, CHENG R, DENG Z, et al. Qualitative analysis of chemical components in Lianhua Qingwen capsule by HPLC-Q Exactive-Orbitrap-MS coupled with GC-MS[J]. J Pharm Anal, 2021, 11(6):709-716.
[22] TAO Y, CAI H, LI W, et al. Ultrafiltration coupled with high-performance liquid chromatography and quadrupole-time-of-flight mass spectrometry for screening lipase binders from different extracts of Dendrobium officinale[J]. Anal Bioanal Chem, 2015, 407(20):6081-6093.
[23] JIANG S, ZHAO X, LIU C, et al. Identification of phenolic compounds in fruits of Ribes stenocarpum Maxim. by UHPLC-QTOF/MS and their hypoglycemic effects in vitro and in vivo[J]. Food Chem, 2021, 344:128568. DOI: 10.1016/j.foodchem.2020.128568.
[24] HUANG Q, ZHANG F, LIU S, et al. Systematic investigation of the pharmacological mechanism for renal protection by the leaves of Eucommia ulmoides Oliver using UPLC-Q-TOF/MS combined with network pharmacology analysis[J]. Biomed Pharmacother, 2021, 140:111735. Doi: 10.1016/j.biopha.2021.111735.
[25] XU K, YANG Y, FENG Z, et al. Six new compounds from Atractylodes lancea and their hepatoprotective activities[J]. Bioorg Med Chem Lett, 2016, 26(21):5187-5192.
[26] ZAN K, GUO L N, MA S C, et al. Analysis of thirty chemical constituents in Tibetan medicine lamiophlomis rotata by UPLC-ESI-TOF MS[J]. Chin Pharm Aff (中国药事), 2018, 32(6):757-763.
[27] ZHANG X Y, XIN G Z, LIU J Q, et al. Identification of chemical constituents of Tripterygium glycosides tablets based on UPLC-QTOF/MS[J]. Acta Chin Med Pharmacol (中医药学报), 2020, 48(9):12-20.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
PDF(1629 KB)
